mouse polyclonal r d systems Search Results


goat anti il 33 polyclonal r d systems  (R&D Systems)
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goat anti mouse ghr polyclonal  (R&D Systems)
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goat anti human ifn γ  (R&D Systems)
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anti human il 1β  (R&D Systems)
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anti human cd4 antibody  (R&D Systems)
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klf4 goat polyclonal  (R&D Systems)
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a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
Klf4 Goat Polyclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti prox1  (R&D Systems)
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a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
Anti Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody anti nrp2  (R&D Systems)
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R&D Systems antibody anti nrp2
a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
Antibody Anti Nrp2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti caspase3  (R&D Systems)
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a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
Anti Caspase3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ret  (R&D Systems)
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R&D Systems anti ret
a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
Anti Ret, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat polyclonal r d systems  (R&D Systems)
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a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
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podocalyxin  (R&D Systems)
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R&D Systems podocalyxin
a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for <t>KLF4,</t> where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.
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Image Search Results


a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for KLF4, where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.

Journal: Nature Genetics

Article Title: The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes

doi: 10.1038/s41588-023-01603-8

Figure Lengend Snippet: a , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by CUT&RUN at RNAPII-enriched (active) and H3K27me3-enriched (PRC2-repressed) promoters. Promoters were grouped based on K-means clustering of RNAPII-S5P and H3K27me3 CUT&Tag reads mapping to a 5 kb window around the TSSs of RefSeq-annotated mESC genes, see Extended Data Fig. . b, c , Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative NANOG foci (smallest fragment within primary peaks called in SL condition), in SL ( b ) and 2i ( c ) mESC culture conditions. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. d , Scatterplots comparing pluripotency TF CUT&RUN and RNAPII-S5P CUT&Tag reads over S5P CUTAC peaks in SL mESCs. e , Immunofluorescent staining comparing pluripotency TF and BRG1 expression in SL versus 2i culture conditions. Cy5-conjugated secondary antibodies were used in all experiment except for KLF4, where Rhodamine red-conjugated antibody was used. DAPI (blue) was used to stain the nucleus in cells. f , Western blot analysis comparing pluripotency TF and BRG1 expression in SL and 2i culture conditions. Equal amounts of extracted total proteins were loaded in each well of 4–20% gradient polyacrylamide SDS electrophoresis gel, and histone H3 signal is used as control to ensure equivalent protein loading. Bar-graph quantifications represent average of two biological replicates with individual data points shown as black dots. Data were normalized to values in SL. g , Heatmaps (bottom) and average plots (top) comparing pluripotency TF occupancy by spike-in calibrated CUT&RUN in SL versus 2i culture conditions. Heatmaps were plotted relative to S5P CUTAC summits showing TF binding at sites of DNA accessibility and sorted by decreasing TF occupancy in SL (CUT&RUN signal). All datasets are representative of at least two biological replicates.

Article Snippet: RNAPII-S5P: rabbit monoclonal (D9N5I, Cell Signaling Technology cat. no. 13523), 1:50; RNAPII-S2P: rabbit monoclonal (E1Z3G, Cell Signaling Technology cat. no. 13499), 1:100; RPB3: rabbit polyclonal (Bethyl Laboratories cat. no. A303-771A, lot no. A303-771A2), 1:100; BRG1: rabbit monoclonal (EPNCIR111A, Abcam cat. no. ab110641), 1:100, for CUT&Tag and CUT&RUN.ChIP, and rabbit polyclonal (Invitrogen cat. no. 720129, lot. no. 2068859), 1:250, for immunofluorescence; H3K4me1: rabbit polyclonal (Abcam cat. no. ab8895, lot no. GR3283237), 1:100; H3K4me3: rabbit polyclonal (Active Motif cat. no. 39915, lot. no. 24118008), 1:100, for CUT&Tag, and rabbit monoclonal (EpiCypher cat. no. 13-0028), 1:100, for CUT&RUN.ChIP; H3K27me3: rabbit monoclonal (C36B11, Cell Signaling Technology cat. no. 9733), 1:100; H3K9me3: rabbit monoclonal (EPR16601, Abcam cat. no. ab176916), 1:100; guinea pig anti-rabbit secondary: Antibodies Online cat. no. ABIN101961, 1:100; isotype control (IgG) for CUT&RUN.ChIP: rabbit monoclonal (EPR25A, Abcam cat. no. ab172730), 1:100; NANOG: rabbit polyclonal (Bethyl Laboratories cat. no. A300-397A, lot no. 3), 1:100; KLF4: goat polyclonal (R&D Systems cat. no. AF3158, lot. no. WRR0719011), 1:100 for CUT&RUN, 1:50 for immunofluorescence; OCT4: rabbit monoclonal (EPR17929, Abcam cat. no. ab181557), 1:100; SOX2: rabbit monoclonal (EPR3131, Abcam cat. no. ab92494), 1:100; CTCF: rabbit monoclonal (EPR7314(B), Abcam cat. no. ab128873), 1:100; rabbit anti-goat secondary: Abcam cat. no. ab6697, 1:100; goat anti-rabbit-Cy5 secondary: Jackson ImmunoResearch cat. no. 111-175-144, 1:500; donkey anti-goat-rhodamine red secondary: Jackson ImmunoResearch cat. no. 705-295-147, 1:250; mouse anti-H3 for western blot: (mAbcam 24834, Abcam cat. no. ab24834), 1:500; IRDye 800CW goat anti-rabbit: LI-COR cat. no. 926-32211, 1:10,000; IRDye 800CW donkey anti-goat: LI-COR cat. no. 926-32214, 1:10,000; IRDye 680RD goat anti-mouse: LI-COR cat. no. 926-68070, 1:10,000.

Techniques: Control, Staining, Expressing, Western Blot, Electrophoresis, Binding Assay